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Zombie Live Dead Stain. The Live cell dye labels intact viable cells green. Unlike titrating antibodies where the goal is to determine the lowest concentration to get a maximal staining index the fixable viability dyes should be titrated down until the live cells are not showing the signature for the dye Figure 6. Zombie Aqua is an amine-reactive fluorescent dye that is non-permeant to live cells but permeant to cells with compromised membranes. LIVEDEAD Fixable Dead Cell Stain kits are fixable viability dyes that distinguish live cells from dead cells based on cell membrane integrity and access to available amines.
The near-IR fluorescent reactive dye has an excitation maximum of 633 nm making it ideal for use with the red or HeNe laser with an emission of 750 nm. About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy Safety How YouTube works Test new features Press Copyright Contact us Creators. The Live Dead assay staining solution is a mixture of two fluorescent dyes that differentially label live and dead cells. Live cell with dye bound to surface primary amines. LIVEDEAD Fixable Dead Cell Stain kits are fixable viability dyes that distinguish live cells from dead cells based on cell membrane integrity and access to available amines. I used Zombie aqua dye fixable viability dye for the first time to stain splenocytes by Flow cytometry.
When using a livedead stain cell populations can be identified more easily and provide a clearer image of the cell viability in your sample.
Locate proteins assay kits reagents custom services. Live cell with dye bound to surface primary amines. Zombie Aqua is an amine-reactive fluorescent dye that is non-permeant to live cells but permeant to cells with compromised membranes. Advertentie Dont compromise your research. Live-or-Dye NucFix Red is a unique cell membrane impermeable dye that specifically stains the nuclei of dead cells. These are then spun down and resuspended in 50ul of MACS buffer before 05ul of livedead stain is added.