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Nov
Stages Of Plant Tissue Culture. Take explants from the parent plant. In vitro material with roots packed approximately 35 plants per tub according to variety. This is the critical stage in tissue culture of plants. The sterilized material is then introduced to the tissue culture.
The new plants can then be placed in soil grown in the normal manner. Tissue Culture Lab. Many types of plants are appropriate for use in the classroom. Plantlets are taken from the vessels and established in soil. Suspension Culture- suspension culture can be defined as a form of culture in which single cells or small aggregates of cells multiply while suspended in an agitated liquid medium. Establishment of an aseptic sterile culture.
This stage can be conducted in the production greenhouse or at the tissue culture labs greenhouse.
The sterilized material is then introduced to the tissue culture. MS medium Whites medium B 5 medium are few culture media that can be obtained from the market for the lab preparation of nutrient medium needed for tissue culture. Minimum order of 100. Tissue Culture Lab. Incubation of Culture 3. Steps in plant tissue culture.