26
May
Ni Nta Binding Buffer. Product is shipped at ambient temperature. This material has excellent handling properties for most scales of batch and column purification. Centrifuge tube for 2 minutes at 700. These phosphate-buffered solutions differ from the Tris-based solutions used in the HisBind Buffer Kit.
Features of HisPur Ni-NTA Magnetic Beads. ③ Column의 10배 vol의 wash buffer buffer B를 흘려준다. This material has excellent handling properties for most scales of batch and column purification. The buffers for Ni-NTA require pH 76 or the His-tags wont bind efficiently to the columnbeads. Choose to go either up or down the pH scale towards the region closest to pH 7 to 8 rather. The Protein 50 kda dimer is bound to Ni-NTA Matrix and then washed with increasing conc.
The buffers for Ni-NTA require pH 76 or the His-tags wont bind efficiently to the columnbeads.
We recommend estimating approximately 75 mg of available binding capacity per ml bed volume of resin therefore 50 µl of Ni-NTA bead slurry yields approximately 40 µg of. 60mg of 28kDa 6xHis -tagged protein from a bacterial source per milliliter of resin. Upon receipt store at 4-8C. Carefully prepared 4X concentrates are included for binding washing and elution according to recommended protocols. This material has excellent handling properties for most scales of batch and column purification. The medium is stable over the pH range 312.