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Fluoro Jade Staining Protocol. Fluoro-jade histological staining protocol. Fluoro-Jade can be used on almost any tissue section. Storage Conditions Store the powder at -20 C avoid light and moisture. 11 rows Incorporating 46-diamidino-2-phenylindole DAPI.
Mix 9 parts distilled water to 1 part Solution C Fluoro-Jade C and place in a Coplin jar in the dark or low light and incubate slides for 10 minutes. To explore a method for combining Fluoro-Jade B FJB staining with immunofluorescent staining in rats with focal cortical infarction. Fluoro-jade The staining solution was prepared from a 001 stock solution for Fluoro-Jade B that was made by adding 10 mg of the dye powder to 100 mL of distilled water. Filter system for visualizing. However Fluoro-Jade B has an even greater specific affinity for degenerating neurons. More relevant content may be added as customer.
Dissolve Fluoro-Jade B powder in distilled water 10 mg powder per in 100 mL water to prepare a 001 stock solution filter through a 045 µm membrane and store at 4C in the dark for up to 3 months.
We strive to provide our customers with a one-stop shop for the entire scientific supplies category. Neutral Red NADPH-diaphorase Iba1-IR and DAPI staining protocols accomplished examination of the cells participating in the acute inflammatory response. A standard Fluoro-Jade B staining protocol was applied to spinal cord sections at 6 12 24 h and 2 4 days postinjection. Fluoro-jade c histological staining protocol. Triple labeling can be accomplished by staining degenerating neurons with Fluoro-Jade C cell nuclei with DAPI and activated astrocytes with GFAP immunofluorescence. Desiccator is recommended when possible.