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Digitonin Vs Triton X 100. Most of the commercially available digitonin preparations contain variable amounts of non-water-soluble contaminants which must be removed prior to use. Triton X-100 or Nonidet P-40 sometimes disrupt protein-protein interactions. Es ist Zeit zu handeln wenn Sie Alkylphenolethoxylat APE-Tenside wie Nonylphenolethoxylate NPEs oder Octylphenolethoxylate OPEs zu denen auch Triton X-100 gehört verwenden. Incubate 37 C for 15 min.
Dilute Triton X-100 110 1 μL Triton X-100 into 9 μL PBS. It is also showed that Triton X-100 is more selective in retaining protein in the detergent phase as evidenced by shift of proteins from detergent to aqueous phase. It is very effective at solubilizing membrane proteins. Digitonin is prepared as a 2 wv stock solution by adding the solid detergent to boiling water stirring for 2 min allowed to stand at room temperature for a week and then filtered Bridges 1977. I am just unclear how the internalised bugs are counted using the saponin method if the cells are still relatively intact. The difference seems to be that Triton X-100 it has a phenolic ring that absorbs UV can interfere in UV spectrometry to determinate proteins at 280 nm.
Cambier et al 1994.
A 15 wv suspension of digitonin. Contact us for support Back to top. Cambier et al 1994. Mansfield Department of Biology University College London Gower Street London WC1E 6BT England. In addition 59kDa was completely partitioned into the aqueous phase of Triton X-114 it was found in both aqueous and detergent portion in the case of Triton X-100 phase partitioning as well as Triton X-114 phase partitioning of protein extracted with Triton X-100. This difference may be greater when used in other applications.